Technologies

The Clinical Genomics Laboratories (CGL) can perform almost any next generation sequencing (NGS) based assay. We use state-of-the-art technologies for all clinical and research work. We also provide end to end NGS bioinformatics solutions for these technologies. Please visit the Bioinformatics page here.

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The HiSeq 2500 system uses sequencing by synthesis (SBS) technology, the most successful and widely adopted next-generation sequencing technology worldwide. SBS technology supports massively parallel sequencing using a proprietary fluorescently labeled reversible terminator method that enables detection of single bases as they are incorporated into growing DNA strands.   It is capable of running in “Rapid Run Mode” with a max read length of 2x250bp with up to 600 million single read or 1.2 billion paired-end reads. The “High Output Run Mode” a max read length of 2x125bp with up to 4 billion single read or 8 billion paired-end reads.

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The MiSeq system uses sequencing by synthesis (SBS) technology, the most successful and widely adopted next-generation sequencing technology worldwide. SBS technology supports massively parallel sequencing using a proprietary fluorescently labeled reversible terminator method that enables detection of single bases as they are incorporated into growing DNA strands.  It is capable of 2x250bp read lengths and with up to 15 million single reads or 30 million paired end reads.

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The Zephyr® G3 NGS Workstation is a benchtop liquid handler designed to automate preparation of libraries for next generation sequencing and address clinical requirements for the physical separation of pre-and post-PCR processes. The simplified user-interface and integrated hardware maximized laboratory productivity while reducing variability resulting from manual pipetting steps. Programs include Library preparation, normalization and qPCR set up.